PDBe 5vza

X-ray diffraction
1.5Å resolution

Pre-catalytic ternary complex of human Polymerase Mu (G433S) mutant with incoming nonhydrolyzable UMPNPP

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
1 distinct polypeptide molecule
3 distinct DNA molecules
Macromolecules (4 distinct):
DNA-directed DNA/RNA polymerase mu Chain: A
Molecule details ›
Chain: A
Length: 354 amino acids
Theoretical weight: 39.87 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9NP87 (Residues: 134-397, 411-494; Coverage: 70%)
Gene names: POLM, polmu
Sequence domains:
DNA (5'-D(*CP*GP*GP*CP*AP*TP*AP*CP*G)-3') Chain: T
Molecule details ›
Chain: T
Length: 9 nucleotides
Theoretical weight: 2.74 KDa
DNA (5'-D(*CP*GP*TP*A)-3') Chain: P
Molecule details ›
Chain: P
Length: 4 nucleotides
Theoretical weight: 1.19 KDa
DNA (5'-D(P*GP*CP*CP*G)-3') Chain: D
Molecule details ›
Chain: D
Length: 4 nucleotides
Theoretical weight: 1.19 KDa

Ligands and Environments


No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P212121
Unit cell:
a: 59.866Å b: 68.838Å c: 110.044Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.17 0.169 0.184
Expression system: Escherichia coli