PDBe 5vpi

X-ray diffraction
1.62Å resolution

Crystal structure of human KRAS G12A mutant in complex with GTP

Released:
Source organism: Homo sapiens
Primary publication:
Structural insight into the rearrangement of the switch I region in GTP-bound G12A K-Ras.
Acta Crystallogr D Struct Biol 73 970-984 (2017)
PMID: 29199977

Function and Biology Details

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
GTPase KRas, N-terminally processed Chains: A, B
Molecule details ›
Chains: A, B
Length: 170 amino acids
Theoretical weight: 19.41 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P01116 (Residues: 1-169; Coverage: 89%)
  • Best match: P01116-2 (Residues: 1-169)
Gene names: KRAS, KRAS2, RASK2
Sequence domains: Ras family

Ligands and Environments

2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 31-ID
Spacegroup: R3
Unit cell:
a: 94.792Å b: 94.792Å c: 121.48Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.188 0.186 0.216
Expression system: Escherichia coli