X-ray diffraction
2.6Å resolution

Electrophilic probes for deciphering substrate recognition by O-GlcNAc transferase


Function and Biology Details

Reactions catalysed:
UDP-N-acetyl-alpha-D-glucosamine + [protein]-L-serine = UDP + [protein]-3-O-(N-acetyl-beta-D-glucosaminyl)-L-serine
ATP + a protein = ADP + a phosphoprotein
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase 110 kDa subunit Chains: A, C
Molecule details ›
Chains: A, C
Length: 723 amino acids
Theoretical weight: 80.97 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
  • Canonical: O15294 (Residues: 323-1041; Coverage: 69%)
  • Best match: O15294-4 (Residues: 1-660)
Gene name: OGT
Sequence domains:
Casein kinase II subunit alpha Chains: B, D
Molecule details ›
Chains: B, D
Length: 14 amino acids
Theoretical weight: 1.4 KDa
Source organism: Homo sapiens
Expression system: Not provided
  • Canonical: P68400 (Residues: 339-352; Coverage: 4%)
Gene names: CK2A1, CSNK2A1

Ligands and Environments

3 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-D
Spacegroup: C2221
Unit cell:
a: 138.734Å b: 200.025Å c: 152.986Å
α: 90° β: 90° γ: 90°
R R work R free
0.251 0.248 0.308
Expression systems:
  • Escherichia coli
  • Not provided