› 5vaw

X-ray diffraction
1.69Å resolution

Fusion of Maltose-binding Protein and PilA from Acinetobacter baumannii AB5075

Released:
DOI: 10.2210/pdb5vaw/pdb
PDB entry 5vaw coloured by chain, front view.
PDB entry 5vaw coloured by chain, side view.
PDB entry 5vaw coloured by chain, top view.
Source organisms:
Primary publication:
The structure of PilA from Acinetobacter baumannii AB5075 suggests a mechanism for functional specialization in Acinetobacter type IV pili.
Ronish LA, Lillehoj E, Fields JK, Sundberg EJ, Piepenbrink KH
J Biol Chem 294 218-230 (2019)
PMID: 30413536

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-101121 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Maltose/maltodextrin-binding periplasmic protein; Prepilin-type N-terminal cleavage/methylation domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 508 amino acids
Theoretical weight: 54.13 KDa
Source organisms: Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P0AEX9 (Residues: 27-392; Coverage: 99%)
  • Canonical: A0A0D5YCX7 (Residues: 26-157; Coverage: 84%)
Gene names: ABUW_0304, EA686_14100, F2P40_00980, JW3994, b4034, malE
Sequence domains:

Ligands and Environments

5 bound ligands:
Ligand MPD 1 x MPD
Ligand NA 1 x NA
Ligand GLU 1 x GLU
Ligand LYS 1 x LYS
Ligand SER 1 x SER
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-D
Spacegroup: P21
Unit cell:
a: 39.557Å b: 103.04Å c: 56.195Å
α: 90° β: 98.95° γ: 90°
R-values:
R R work R free
0.21 0.209 0.242
Expression system: Escherichia coli BL21

Quick links

Citations

9 review citations

Acinetobacter baumannii: An Ancient Commensal with Weapons of a Pathogen.
Sarshar et al. (2021)
8 more

2 mentions without citation

The structure of PilA from Acinetobacter baumannii AB5075 suggests a mechanism for functional specialization in Acinetobacter type IV pili.
Ronish et al. (2019)
1 more