PDBe 5v8f

Electron Microscopy
3.9Å resolution

Structural basis of MCM2-7 replicative helicase loading by ORC-Cdc6 and Cdt1

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero 16-mer (preferred)
Entry contents:
14 distinct polypeptide molecules
2 distinct DNA molecules
Macromolecules (16 distinct):
DNA replication licensing factor MCM2 Chain: 2
Molecule details ›
Chain: 2
Length: 868 amino acids
Theoretical weight: 98.91 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: P29469 (Residues: 1-868; Coverage: 100%)
Gene names: MCM2, YBL023C, YBL0438
Sequence domains:
DNA replication licensing factor MCM3 Chain: 3
Molecule details ›
Chain: 3
Length: 971 amino acids
Theoretical weight: 107.65 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: P24279 (Residues: 1-971; Coverage: 100%)
Gene names: MCM3, SYGP-ORF23, YEL032W
Sequence domains:
DNA replication licensing factor MCM4 Chain: 4
Molecule details ›
Chain: 4
Length: 933 amino acids
Theoretical weight: 105.14 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: P30665 (Residues: 1-933; Coverage: 100%)
Gene names: CDC54, HCD21, MCM4, YP9531.13, YPR019W
Sequence domains:
Minichromosome maintenance protein 5 Chain: 5
Molecule details ›
Chain: 5
Length: 775 amino acids
Theoretical weight: 86.51 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: P29496 (Residues: 1-775; Coverage: 100%)
Gene names: CDC46, L9328.1, MCM5, YLR274W
Sequence domains:
DNA replication licensing factor MCM6 Chain: 6
Molecule details ›
Chain: 6
Length: 1017 amino acids
Theoretical weight: 113.11 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: P53091 (Residues: 1-1017; Coverage: 100%)
Gene names: MCM6, YGL201C
Sequence domains:
DNA replication licensing factor MCM7 Chain: 7
Molecule details ›
Chain: 7
Length: 800 amino acids
Theoretical weight: 90.31 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: P38132 (Residues: 1-800; Coverage: 95%)
Gene names: CDC47, MCM7, YBR1441, YBR202W
Sequence domains:
Cell division cycle protein CDT1 Chain: 8
Molecule details ›
Chain: 8
Length: 604 amino acids
Theoretical weight: 68.49 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: P47112 (Residues: 1-604; Coverage: 100%)
Gene names: CDT1, J1641, SID2, TAH11, YJR046W
Cell division control protein 6 Chain: 9
Molecule details ›
Chain: 9
Length: 513 amino acids
Theoretical weight: 58.11 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: P09119 (Residues: 1-513; Coverage: 100%)
Gene names: CDC6, J0347, YJL194W
Sequence domains:
Origin recognition complex subunit 1 Chain: A
Molecule details ›
Chain: A
Length: 913 amino acids
Theoretical weight: 104.43 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: P54784 (Residues: 1-913; Coverage: 100%)
Gene names: ORC1, YML065W
Sequence domains:
Origin recognition complex subunit 2 Chain: B
Molecule details ›
Chain: B
Length: 620 amino acids
Theoretical weight: 71.34 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: P32833 (Residues: 1-620; Coverage: 100%)
Gene names: ORC2, RRR1, SIR5, YBR0523, YBR060C
Sequence domains: Origin recognition complex subunit 2
Origin recognition complex subunit 3 Chain: C
Molecule details ›
Chain: C
Length: 616 amino acids
Theoretical weight: 72.16 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: P54790 (Residues: 1-616; Coverage: 100%)
Gene names: L1365, OAF1, OIF1, ORC3, YLL004W
Sequence domains: Origin recognition complex winged helix C-terminal
Origin recognition complex subunit 4 Chain: D
Molecule details ›
Chain: D
Length: 529 amino acids
Theoretical weight: 60.77 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: P54791 (Residues: 1-529; Coverage: 100%)
Gene names: ORC4, P9325.5, YPR162C
Sequence domains: Origin recognition complex (ORC) subunit 4 C-terminus
Origin recognition complex subunit 5 Chain: E
Molecule details ›
Chain: E
Length: 479 amino acids
Theoretical weight: 55.35 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: P50874 (Residues: 1-479; Coverage: 100%)
Gene names: N0834, ORC5, YNL261W
Sequence domains:
Origin recognition complex subunit 6 Chain: F
Molecule details ›
Chain: F
Length: 435 amino acids
Theoretical weight: 50.37 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: P38826 (Residues: 1-435; Coverage: 100%)
Gene names: AAP1, ORC6, YHR118C
Sequence domains: Origin recognition complex subunit 6 (ORC6)
DNA (39-MER) Chain: M
Molecule details ›
Chain: M
Length: 39 nucleotides
Theoretical weight: 11.97 KDa
DNA (39-MER) Chain: N
Molecule details ›
Chain: N
Length: 39 nucleotides
Theoretical weight: 12.04 KDa

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.9Å
Relevant EMDB volumes: EMD-8540
Expression system: Saccharomyces cerevisiae