› 5v36

X-ray diffraction
1.88Å resolution

1.88 Angstrom Resolution Crystal Structure of Glutathione Reductase from Streptococcus mutans UA159 in Complex with FAD

Released:
DOI: 10.2210/pdb5v36/pdb
PDB entry 5v36 coloured by chain, front view.
PDB entry 5v36 coloured by chain, side view.
PDB entry 5v36 coloured by chain, top view.
Source organism: Streptococcus mutans UA159
Entry authors: Minasov G, Shuvalova L, Dubrovska I, Kiryukhina O, Grimshaw S, Kwon K, Anderson WF, Center for Structural Genomics of Infectious Diseases (CSGID)

Function and Biology Details

Reaction catalysed: 
2 glutathione + NADP(+) = glutathione disulfide + NADPH
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
homo dimer (preferred)
homo tetramer
Assembly name:
PDBe Complex ID:
PDB-CPX-184229 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glutathione reductase Chains: A, B
Molecule details ›
Chains: A, B
Length: 453 amino acids
Theoretical weight: 49.31 KDa
Source organism: Streptococcus mutans UA159
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q8DUR5 (Residues: 1-450; Coverage: 100%)
Gene names: SMU_838, gshR
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand FAD 2 x FAD
6 bound ligands:
Ligand BME 2 x BME
Ligand CL 8 x CL
Ligand SO4 30 x SO4
Ligand GOL 5 x GOL
Ligand EPE 1 x EPE
Ligand BDF 1 x BDF
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-F
Spacegroup: P6522
Unit cell:
a: 126.333Å b: 126.333Å c: 247.876Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.144 0.143 0.17
Expression system: Escherichia coli BL21(DE3)

Quick links