› 5v0o

X-ray diffraction
2.7Å resolution

RNA hairpin structure containing 2-MeImpG analogue bound

Released:
DOI: 10.2210/pdb5v0o/pdb
PDB entry 5v0o coloured by chain, front view.
PDB entry 5v0o coloured by chain, side view.
PDB entry 5v0o coloured by chain, top view.
Source organism: synthetic construct
Primary publication:
Structural Rationale for the Enhanced Catalysis of Nonenzymatic RNA Primer Extension by a Downstream Oligonucleotide.
Zhang W, Tam CP, Zhou L, Oh SS, Wang J, Szostak JW
J Am Chem Soc 140 2829-2840 (2018)
PMID: 29411978

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-196993 (preferred)
Entry contents:
2 distinct RNA molecules
Macromolecules (2 distinct):
RNA (25-MER) Chain: A
Molecule details ›
Chain: A
Length: 25 nucleotides
Theoretical weight: 8.16 KDa
Source organism: synthetic construct
Expression system: Not provided
RNA (5'-R(*GP*CP*AP*CP*CP*UP*CP*A)-3') Chain: B
Molecule details ›
Chain: B
Length: 8 nucleotides
Theoretical weight: 2.49 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand 8OS 1 x 8OS
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.2
Spacegroup: P43212
Unit cell:
a: 44.747Å b: 44.747Å c: 154.198Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.22 0.218 0.277
Expression system: Not provided

Quick links

Citations

2 review citations

Exploring the Emergence of RNA Nucleosides and Nucleotides on the Early Earth.
Biscans A. (2018)
1 more