5v0d

X-ray diffraction
2.63Å resolution

Crystal structure of human exonuclease 1 Exo1 (WT) in complex with 5' flap DNA (f2II)

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
1 distinct polypeptide molecule
3 distinct DNA molecules
Macromolecules (4 distinct):
Exonuclease 1 Chain: Z
Molecule details ›
Chain: Z
Length: 358 amino acids
Theoretical weight: 40.35 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9UQ84 (Residues: 1-352; Coverage: 42%)
Gene names: EXO1, EXOI, HEX1
Sequence domains:
DNA (5'-D(*CP*GP*CP*TP*AP*GP*TP*AP*CP*TP*CP*A)-3') Chain: A
Molecule details ›
Chain: A
Length: 13 nucleotides
Theoretical weight: 3.93 KDa
Source organism: synthetic construct
Expression system: Not provided
DNA (5'-D(P*GP*TP*AP*CP*TP*AP*GP*CP*G)-3') Chain: B
Molecule details ›
Chain: B
Length: 9 nucleotides
Theoretical weight: 2.76 KDa
Source organism: synthetic construct
Expression system: Not provided
DNA (5'-D(P*GP*TP*AP*CP*TP*AP*GP*CP*G)-3') Chain: E
Molecule details ›
Chain: E
Length: 3 nucleotides
Theoretical weight: 862 Da
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P43212
Unit cell:
a: 72.976Å b: 72.976Å c: 180.612Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.208 0.205 0.237
Expression systems:
  • Escherichia coli
  • Not provided