X-ray diffraction
1.7Å resolution

Crystal structure of macrolide 2'-phosphotransferase MphH from Brachybacterium faecium in complex with azithromycin


Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
APH domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 300 amino acids
Theoretical weight: 32.21 KDa
Source organism: Brachybacterium faecium DSM 4810
Expression system: Escherichia coli BL21(DE3)
  • Canonical: C7MEP1 (Residues: 1-298; Coverage: 100%)
Gene name: Bfae_22410
Sequence domains: Phosphotransferase enzyme family

Ligands and Environments

3 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-G
Spacegroup: P21
Unit cell:
a: 55.05Å b: 81.21Å c: 71.34Å
α: 90° β: 96.83° γ: 90°
R R work R free
0.192 0.19 0.232
Expression system: Escherichia coli BL21(DE3)