X-ray diffraction
1.72Å resolution

Crystal structure of macrolide 2'-phosphotransferase MphH from Brachybacterium faecium in complex with GDP


Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
APH domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 299 amino acids
Theoretical weight: 32.29 KDa
Source organism: Brachybacterium faecium DSM 4810
Expression system: Escherichia coli
  • Canonical: C7MEP1 (Residues: 1-298; Coverage: 100%)
Gene name: Bfae_22410
Sequence domains: Phosphotransferase enzyme family

Ligands and Environments

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-F
Spacegroup: P212121
Unit cell:
a: 49.556Å b: 77.002Å c: 94.409Å
α: 90° β: 90° γ: 90°
R R work R free
0.166 0.164 0.207
Expression system: Escherichia coli