5umx

X-ray diffraction
1.59Å resolution

Crystal structure of TnmS3 in complex with riboflavin

Released:
Source organism: Streptomyces sp. CB03234
Entry authors: Chang CY, Chang C, Nocek B, Rudolf JD, Joachimiak A, Phillips Jr GN, Shen B, Enzyme Discovery for Natural Product Biosynthesis (NatPro), Midwest Center for Structural Genomics (MCSG)

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
VOC domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 140 amino acids
Theoretical weight: 15.15 KDa
Source organism: Streptomyces sp. CB03234
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A125SA29 (Residues: 1-124; Coverage: 100%)
Gene names: AMK26_32110, tnmS3
Sequence domains: Glyoxalase/Bleomycin resistance protein/Dioxygenase superfamily
Structure domains: 2,3-Dihydroxybiphenyl 1,2-Dioxygenase, domain 1

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P212121
Unit cell:
a: 45.059Å b: 60.915Å c: 85.659Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.166 0.165 0.193
Expression system: Escherichia coli BL21(DE3)