› 5um2

X-ray diffraction
1.14Å resolution

Functional and structural characterization of a Sulfate-binding protein (Sbp) from Xanthomonas citri

Released:
DOI: 10.2210/pdb5um2/pdb
PDB entry 5um2 coloured by chain, front view.
PDB entry 5um2 coloured by chain, side view.
PDB entry 5um2 coloured by chain, top view.
Source organism: Xanthomonas citri pv. citri str. 306
Primary publication:
Sulfate-Binding Protein (Sbp) from Xanthomonas citri: Structure and Functional Insights.
Pereira CT, Roesler C, Faria JN, Fessel MR, Balan A
Mol Plant Microbe Interact 30 578-588 (2017)
PMID: 28562158

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-241437 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
ABC transporter sulfate binding protein Chain: A
Molecule details ›
Chain: A
Length: 334 amino acids
Theoretical weight: 37.46 KDa
Source organism: Xanthomonas citri pv. citri str. 306
Expression system: Escherichia coli BL21(DE3)
Structure domains: Periplasmic binding protein-like II

Ligands and Environments

2 bound ligands:
Ligand SO4 2 x SO4
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I02
Spacegroup: C2
Unit cell:
a: 183.06Å b: 54.54Å c: 34.2Å
α: 90° β: 98.9° γ: 90°
R-values:
R R work R free
0.122 0.121 0.139
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 review citation

The role of solute binding proteins in signal transduction.
Matilla et al. (2021)
3 other articles

1 mention without citation

A Non-Invasive Tool for Real-Time Measurement of Sulfate in Living Cells.
Fatima et al. (2020)