› 5uk9

X-ray diffraction
1.89Å resolution

Wild-type K-Ras(GCP) pH 6.5

Released:
DOI: 10.2210/pdb5uk9/pdb
PDB entry 5uk9 coloured by chain, front view.
PDB entry 5uk9 coloured by chain, side view.
PDB entry 5uk9 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
K-Ras Populates Conformational States Differently from Its Isoform H-Ras and Oncogenic Mutant K-RasG12D.
Parker JA, Volmar AY, Pavlopoulos S, Mattos C
Structure 26 810-820.e4 (2018)
PMID: 29706533

Function and Biology Details

Reaction catalysed: 
GTP + H(2)O = GDP + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-133991 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
GTPase KRas, N-terminally processed Chains: A, B
Molecule details ›
Chains: A, B
Length: 166 amino acids
Theoretical weight: 18.88 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P01116 (Residues: 1-166; Coverage: 88%)
Gene names: KRAS, KRAS2, RASK2
Sequence domains: Ras family
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

4 bound ligands:
Ligand GDP 1 x GDP
Ligand MG 2 x MG
Ligand GOL 3 x GOL
Ligand GCP 1 x GCP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007 HF
Spacegroup: P212121
Unit cell:
a: 39.107Å b: 87.553Å c: 93.692Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.167 0.164 0.206
Expression system: Escherichia coli

Quick links

Citations

6 review citations

RAS variant signalling.
Mo et al. (2018)
5 more

8 mentions without citation

The K-Ras, N-Ras, and H-Ras Isoforms: Unique Conformational Preferences and Implications for Targeting Oncogenic Mutants.
Parker et al. (2018)
7 more