› 5ujc

X-ray diffraction
1.35Å resolution

Crystal structure of a C.elegans B12-trafficking protein CblC, a human MMACHC homologue

Released:
DOI: 10.2210/pdb5ujc/pdb
PDB entry 5ujc coloured by chain, front view.
PDB entry 5ujc coloured by chain, side view.
PDB entry 5ujc coloured by chain, top view.
Source organism: Caenorhabditis elegans
Primary publication:
Coordination chemistry controls the thiol oxidase activity of the B12-trafficking protein CblC.
Li Z, Shanmuganathan A, Ruetz M, Yamada K, Lesniak NA, Kräutler B, Brunold TC, Koutmos M, Banerjee R
J Biol Chem 292 9733-9744 (2017)
PMID: 28442570

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-182231 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
MMACHC-like protein Chain: A
Molecule details ›
Chain: A
Length: 270 amino acids
Theoretical weight: 31.35 KDa
Source organism: Caenorhabditis elegans
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
UniProt:
  • Canonical: Q7Z144 (Residues: 1-264; Coverage: 97%)
Gene names: ZK546.17, cblc-1
Sequence domains: Methylmalonic aciduria and homocystinuria type C family

Ligands and Environments

4 bound ligands:
Ligand COB 1 x COB
Ligand TLA 2 x TLA
Ligand TAR 1 x TAR
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-B
Spacegroup: P212121
Unit cell:
a: 53.008Å b: 66.089Å c: 92.826Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.134 0.132 0.163
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'

Quick links

Citations

2 review citations

Altered Redox Homeostasis in Branched-Chain Amino Acid Disorders, Organic Acidurias, and Homocystinuria.
Richard et al. (2018)
1 more

2 mentions without citation

Coordination chemistry controls the thiol oxidase activity of the B12-trafficking protein CblC.
Li et al. (2017)
1 more