› 5uj1

X-ray diffraction
2.15Å resolution

Crystal structure of Mycobacterium tuberculosis Topoisomerase I at 2.15A resolution limit

Released:
DOI: 10.2210/pdb5uj1/pdb
PDB entry 5uj1 coloured by chain, front view.
PDB entry 5uj1 coloured by chain, side view.
PDB entry 5uj1 coloured by chain, top view.
Source organism: Mycobacterium tuberculosis
Primary publication:
Investigating mycobacterial topoisomerase I mechanism from the analysis of metal and DNA substrate interactions at the active site.
Cao N, Tan K, Annamalai T, Joachimiak A, Tse-Dinh YC
Nucleic Acids Res 46 7296-7308 (2018)
PMID: 29905859

Function and Biology Details

Reaction catalysed: 
ATP-independent breakage of single-stranded DNA, followed by passage and rejoining
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-161458 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
DNA topoisomerase 1 Chain: A
Molecule details ›
Chain: A
Length: 706 amino acids
Theoretical weight: 77.84 KDa
Source organism: Mycobacterium tuberculosis
Expression system: Escherichia coli
UniProt:
  • Canonical: P9WG49 (Residues: 2-704; Coverage: 75%)
Gene names: MTCY15C10.06, Rv3646c, topA
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand SO4 1 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P212121
Unit cell:
a: 63.783Å b: 93.588Å c: 140.571Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.17 0.168 0.21
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Unravelling the mechanisms of Type 1A topoisomerases using single-molecule approaches.
Spakman et al. (2021)
2 more

3 mentions without citation

Mechanism of Type IA Topoisomerases.
Dasgupta et al. (2020)
2 more