› 5uhu

Solution NMR

Solution conformation of cytochrome P450 MycG with mycinamicin IV bound

Released:
DOI: 10.2210/pdb5uhu/pdb
PDB entry 5uhu coloured by chain, front view.
PDB entry 5uhu coloured by chain, side view.
PDB entry 5uhu coloured by chain, top view.
Source organism: Micromonospora griseorubida
Primary publication:
Solution Conformations and Dynamics of Substrate-Bound Cytochrome P450 MycG.
Tietz DR, Podust LM, Sherman DH, Pochapsky TC
Biochemistry 56 2701-2714 (2017)
PMID: 28488849

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-176804 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Mycinamicin IV hydroxylase/epoxidase Chain: A
Molecule details ›
Chain: A
Length: 397 amino acids
Theoretical weight: 44.39 KDa
Source organism: Micromonospora griseorubida
Expression system: Escherichia coli
UniProt:
  • Canonical: Q59523 (Residues: 1-397; Coverage: 100%)
Gene name: mycG
Sequence domains: Cytochrome P450
Structure domains: Cytochrome P450

Ligands and Environments

2 bound ligands:
Ligand HEM 1 x HEM
Ligand MIV 1 x MIV
No modified residues

Experiments and Validation Details

Entry percentile scores
Chemical shift assignment: 23%
Expression system: Escherichia coli

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Citations

2 review citations

An overview of the cytochrome P450 enzymes that catalyze the same-site multistep oxidation reactions in biotechnologically relevant selected actinomycete strains.
Iizaka et al. (2021)
1 more

5 mentions without citation

A dynamic understanding of cytochrome P450 structure and function through solution NMR.
Pochapsky TC. (2021)
4 more