5uh7

X-ray diffraction
2.2Å resolution

Crystal structure of beta'MtbSI of Mycobacterium tuberculosis RNA polymerase

Released:

Function and Biology Details

Reaction catalysed:
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
DNA-directed RNA polymerase subunit beta' Chains: A, B
Molecule details ›
Chains: A, B
Length: 169 amino acids
Theoretical weight: 19.51 KDa
Source organism: Mycobacterium tuberculosis H37Rv
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
UniProt:
  • Canonical: P9WGY7 (Residues: 124-271; Coverage: 11%)
Gene names: MTCI376.07c, Rv0668, rpoC

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P21
Unit cell:
a: 40.753Å b: 97.5Å c: 53.741Å
α: 90° β: 93.3° γ: 90°
R-values:
R R work R free
0.222 0.22 0.246
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'