› 5ucq

X-ray diffraction
1.4Å resolution

The structure of archaeal Inorganic Pyrophosphatase in complex with pyrophosphate

Released:
DOI: 10.2210/pdb5ucq/pdb
PDB 5ucq coloured by chain and viewed from the front.
PDB 5ucq coloured by chain and viewed from the side.
PDB 5ucq coloured by chain and viewed from the top.
Source organism: Thermococcus thioreducens
Entry authors: Inoguchi N, Hughes RC, Meehan EJ, Ng JD

Function and Biology Details

Reaction catalysed: 
Diphosphate + H(2)O = 2 phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Inorganic pyrophosphatase Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 178 amino acids
Theoretical weight: 20.88 KDa
Source organism: Thermococcus thioreducens
Expression system: Escherichia coli
UniProt:
  • Canonical: H0USY5 (Residues: 1-178; Coverage: 100%)
Gene names: A3L14_01550, SAMN05216170_1971, ppa
Sequence domains: Inorganic pyrophosphatase
Structure domains: Inorganic pyrophosphatase

Ligands and Environments

5 bound ligands:
Ligand CA 9 x CA
Ligand EDO 9 x EDO
Ligand MRD 1 x MRD
Ligand POP 3 x POP
Ligand NA 1 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-BM
Spacegroup: P3221
Unit cell:
a: 100.161Å b: 100.161Å c: 97.801Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.11 0.109 0.14
Expression system: Escherichia coli

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