5ucq

X-ray diffraction
1.4Å resolution

The structure of archaeal Inorganic Pyrophosphatase in complex with pyrophosphate

Released:
Source organism: Thermococcus thioreducens
Entry authors: Inoguchi N, Hughes RC, Meehan EJ, Ng JD

Function and Biology Details

Reaction catalysed:
Diphosphate + H(2)O = 2 phosphate
Biochemical function:
Cellular component:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Inorganic pyrophosphatase Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 178 amino acids
Theoretical weight: 20.88 KDa
Source organism: Thermococcus thioreducens
Expression system: Escherichia coli
UniProt:
  • Canonical: H0USY5 (Residues: 1-178; Coverage: 100%)
Gene names: A3L14_01550, SAMN05216170_1971, ppa
Sequence domains: Inorganic pyrophosphatase
Structure domains: Inorganic pyrophosphatase

Ligands and Environments

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-BM
Spacegroup: P3221
Unit cell:
a: 100.161Å b: 100.161Å c: 97.801Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.11 0.109 0.14
Expression system: Escherichia coli