› 5uce

Solution NMR

Solution NMR structure of the major species of DANCER-2, a dynamic and natively folded pentamutant of the B1 domain of streptococcal protein G (GB1)

Released:
DOI: 10.2210/pdb5uce/pdb
PDB entry 5uce coloured by chain, ensemble of 10 models, front view.
PDB entry 5uce coloured by chain, ensemble of 10 models, side view.
PDB entry 5uce coloured by chain, ensemble of 10 models, top view.
Source organism: Streptococcus sp. GX7805
Primary publication:
Rational design of proteins that exchange on functional timescales.
Davey JA, Damry AM, Goto NK, Chica RA
Nat Chem Biol 13 1280-1285 (2017)
PMID: 29058725

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-148791 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Immunoglobulin G-binding protein G Chain: A
Molecule details ›
Chain: A
Length: 56 amino acids
Theoretical weight: 6.23 KDa
Source organism: Streptococcus sp. GX7805
Expression system: Escherichia coli
UniProt:
  • Canonical: P19909 (Residues: 373-427; Coverage: 10%)
Gene name: spg
Sequence domains: B domain
Structure domains: Ubiquitin-like (UB roll)

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Chemical shift assignment: 86%
Refinement method: simulated annealing
Expression system: Escherichia coli

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Citations

14 review citations

Advances in protein structure prediction and design.
Kuhlman et al. (2019)
13 more

1 mention without citation

The stability and dynamics of computationally designed proteins.
Gonzalez et al. (2022)