5uc7

X-ray diffraction
1.84Å resolution

Crystal structure of BioA / 7,8-diaminopelargonic acid aminotransferase / DAPA synthase from Citrobacter rodentium, PLP complex

Released:
Source organism: environmental samples
Entry authors: Stogios PJ, Evdokimova E, Di Leo R, Savchenko A, Anderson WF, Center for Structural Genomics of Infectious Diseases (CSGID)

Function and Biology Details

Reaction catalysed:
S-adenosyl-L-methionine + 8-amino-7-oxononanoate = S-adenosyl-4-methylthio-2-oxobutanoate + 7,8-diaminononanoate
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Adenosylmethionine-8-amino-7-oxononanoate aminotransferase Chain: A
Molecule details ›
Chain: A
Length: 431 amino acids
Theoretical weight: 47.71 KDa
Source organism: environmental samples
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: D2TPI3 (Residues: 1-429; Coverage: 100%)
Gene names: ROD_07751, bioA
Sequence domains: Aminotransferase class-III
Structure domains:

Ligands and Environments

1 bound ligand:
1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-G
Spacegroup: P21212
Unit cell:
a: 75.369Å b: 88.306Å c: 63.274Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.154 0.152 0.198
Expression system: Escherichia coli BL21(DE3)