› 5uai

X-ray diffraction
2.75Å resolution

Crystal structure of Methionyl-tRNA formyltransferase from Pseudomonas aeruginosa

Released:
DOI: 10.2210/pdb5uai/pdb
PDB entry 5uai coloured by chain, front view.
PDB entry 5uai coloured by chain, side view.
PDB entry 5uai coloured by chain, top view.
Source organism: Pseudomonas aeruginosa PAO1
Entry author: Seattle Structural Genomics Center for Infectious Disease (SSGCID)

Function and Biology Details

Reaction catalysed: 
10-formyltetrahydrofolate + L-methionyl-tRNA(fMet) = tetrahydrofolate + N-formylmethionyl-tRNA(fMet)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-131443 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Methionyl-tRNA formyltransferase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 322 amino acids
Theoretical weight: 34.1 KDa
Source organism: Pseudomonas aeruginosa PAO1
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O85732 (Residues: 1-314; Coverage: 100%)
Gene names: PA0018, fmt
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand EDO 1 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-G
Spacegroup: P21212
Unit cell:
a: 113.92Å b: 109.06Å c: 109.93Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.171 0.167 0.234
Expression system: Escherichia coli BL21(DE3)

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