PDBe 5u4j

Electron Microscopy
3.7Å resolution

Structural Basis of Co-translational Quality Control by ArfA and RF2 Bound to Ribosome

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero decamer (preferred)
Entry contents:
6 distinct polypeptide molecules
4 distinct RNA molecules
Macromolecules (10 distinct):
30S ribosomal protein S3 Chain: c
Molecule details ›
Chain: c
Length: 233 amino acids
Theoretical weight: 26.03 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P0A7V3 (Residues: 1-233; Coverage: 100%)
Gene names: JW3276, b3314, rpsC
Sequence domains:
30S ribosomal protein S4 Chain: d
Molecule details ›
Chain: d
Length: 206 amino acids
Theoretical weight: 23.51 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P0A7V8 (Residues: 1-206; Coverage: 100%)
Gene names: JW3258, b3296, ramA, rpsD
Sequence domains:
30S ribosomal protein S5 Chain: e
Molecule details ›
Chain: e
Length: 167 amino acids
Theoretical weight: 17.63 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P0A7W1 (Residues: 1-167; Coverage: 100%)
Gene names: JW3265, b3303, rpsE, spc
Sequence domains:
30S ribosomal protein S12 Chain: l
Molecule details ›
Chain: l
Length: 124 amino acids
Theoretical weight: 13.77 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P0A7S3 (Residues: 1-124; Coverage: 100%)
Gene names: JW3304, b3342, rpsL, strA
Sequence domains: Ribosomal protein S12/S23
Peptide chain release factor RF2 Chain: v
Molecule details ›
Chain: v
Length: 383 amino acids
Theoretical weight: 43.3 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P07012 (Residues: 1-365; Coverage: 100%)
Gene names: JW5847, b2891, prfB, supK
Sequence domains:
Alternative ribosome-rescue factor A Chain: w
Molecule details ›
Chain: w
Length: 57 amino acids
Theoretical weight: 6.51 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P36675 (Residues: 1-55; Coverage: 76%)
Gene names: JW3253, arfA, b4550, yhdL
Sequence domains: Alternative ribosome-rescue factor A
16S ribosomal RNA Chain: a
Molecule details ›
Chain: a
Length: 1533 nucleotides
Theoretical weight: 497.08 KDa
Sequence domains: Bacterial small subunit ribosomal RNA
23S ribosomal RNA Chain: A
Molecule details ›
Chain: A
Length: 2904 nucleotides
Theoretical weight: 941.79 KDa
Sequence domains: Bacterial large subunit ribosomal RNA
Messenger RNA Chain: z
Molecule details ›
Chain: z
Length: 18 nucleotides
Theoretical weight: 5.92 KDa
P-site tRNA fMet Chain: x
Molecule details ›
Chain: x
Length: 77 nucleotides
Theoretical weight: 24.79 KDa
Sequence domains: tRNA

Ligands and Environments

Experiments and Validation Details

Entry percentile scores
Resolution: 3.7Å
Relevant EMDB volumes: EMD-8506
Expression system: Escherichia coli