PDBe 5u0j

X-ray diffraction
1.72Å resolution

C-terminal ankyrin repeats from human kidney-type glutaminase (KGA) - monoclinic crystal form

Released:

Function and Biology Details

Reaction catalysed:
L-glutamine + H(2)O = L-glutamate + NH(3)
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glutaminase kidney isoform, mitochondrial 65 kDa chain Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 140 amino acids
Theoretical weight: 15.81 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: O94925 (Residues: 551-669; Coverage: 18%)
Gene names: GLS, GLS1, KIAA0838
Sequence domains: Ankyrin repeats (3 copies)

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL12-2
Spacegroup: P21
Unit cell:
a: 76.49Å b: 47.18Å c: 115.698Å
α: 90° β: 90.09° γ: 90°
R-values:
R R work R free
0.185 0.184 0.222
Expression system: Escherichia coli