5tz2

X-ray diffraction
2.3Å resolution

Crystal structure of human CD47 ECD bound to Fab of C47B222

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (4 distinct):
C47B222 Fab Heavy Chain Chain: H
Molecule details ›
Chain: H
Length: 229 amino acids
Theoretical weight: 24.64 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
Structure domains: Immunoglobulins
C47B222 Fab Light Chain Chain: L
Molecule details ›
Chain: L
Length: 214 amino acids
Theoretical weight: 23.41 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
Structure domains: Immunoglobulins
Leukocyte surface antigen CD47 Chain: C
Molecule details ›
Chain: C
Length: 129 amino acids
Theoretical weight: 14.76 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: Q08722 (Residues: 19-141; Coverage: 40%)
Gene names: CD47, MER6
Sequence domains: CD47 immunoglobulin-like domain
Structure domains: Immunoglobulins

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 17-ID
Spacegroup: P21
Unit cell:
a: 60.567Å b: 72.934Å c: 72.698Å
α: 90° β: 108.97° γ: 90°
R-values:
R R work R free
0.204 0.201 0.255
Expression system: Homo sapiens