5tyx

X-ray diffraction
1.95Å resolution

DNA Polymerase Mu Product Complex, Mn2+ (15 min)

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
1 distinct polypeptide molecule
3 distinct DNA molecules
Macromolecules (4 distinct):
DNA-directed DNA/RNA polymerase mu Chain: A
Molecule details ›
Chain: A
Length: 356 amino acids
Theoretical weight: 40.05 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9NP87 (Residues: 132-494; Coverage: 71%)
Gene names: POLM, polmu
Sequence domains:
Structure domains:
DNA (5'-D(*CP*GP*GP*CP*AP*TP*AP*CP*G)-3') Chain: T
Molecule details ›
Chain: T
Length: 9 nucleotides
Theoretical weight: 2.74 KDa
Source organism: unidentified
Expression system: Not provided
DNA (5'-D(*CP*GP*TP*AP*T)-3') Chain: P
Molecule details ›
Chain: P
Length: 5 nucleotides
Theoretical weight: 1.5 KDa
Source organism: unidentified
Expression system: Not provided
DNA (5'-D(P*GP*CP*CP*G)-3') Chain: D
Molecule details ›
Chain: D
Length: 4 nucleotides
Theoretical weight: 1.19 KDa
Source organism: unidentified
Expression system: Not provided

Ligands and Environments

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007 HF
Spacegroup: P212121
Unit cell:
a: 59.908Å b: 68.736Å c: 109.962Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.187 0.185 0.216
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided