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DNA Polymerase Mu Reactant Complex, Mn2+ (4 min)

X-ray diffraction
2.048Å resolution
PDB entry 5tyu coloured by chain, front view.
PDB entry 5tyu coloured by chain, side view.
PDB entry 5tyu coloured by chain, top view.
Source organisms:
Assembly composition:
hetero tetramer (preferred)
Entry contents: 1 distinct polypeptide molecule
3 distinct DNA molecules

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Assemblies

Assembly 1 (preferred)
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Assembly Name: DNA-directed DNA/RNA polymerase mu and DNA
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Multimeric state: hetero tetramer
Accessible surface area: 15901.91 Å2
Buried surface area: 6867.14 Å2
Dissociation area: 550.34 Å2
Dissociation energy (ΔGdiss): 8.76 kcal/mol
Dissociation entropy (TΔSdiss): 7.42 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-119175

Macromolecules

DNA-directed DNA/RNA polymerase mu
Chain: A
Length: 356 amino acids
Theoretical weight: 40.05 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9NP87 (Residues: 132-494; Coverage: 71%)
Gene names: POLM, polmu
Pfam:
InterPro:
CATH:
DNA-directed DNA/RNA polymerase mu in PDB entry 5tyu, assembly 1, front view.
DNA-directed DNA/RNA polymerase mu in PDB entry 5tyu, assembly 1, side view.
DNA-directed DNA/RNA polymerase mu in PDB entry 5tyu, assembly 1, top view.
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DNA chain T
Name: DNA (5'-D(*CP*GP*GP*CP*AP*TP*AP*CP*G)-3')
Representative chains: T
Source organism: unidentified [32644]
Expression system: Not provided
Length: 9 nucleotides
Theoretical weight: 2.74 KDa
DNA (5'-D(*CP*GP*GP*CP*AP*TP*AP*CP*G)-3') in PDB entry 5tyu, assembly 1, front view.
DNA (5'-D(*CP*GP*GP*CP*AP*TP*AP*CP*G)-3') in PDB entry 5tyu, assembly 1, side view.
DNA (5'-D(*CP*GP*GP*CP*AP*TP*AP*CP*G)-3') in PDB entry 5tyu, assembly 1, top view.
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DNA chain P
Name: DNA (5'-D(*CP*GP*TP*AP*T)-3')
Representative chains: P
Source organism: unidentified [32644]
Expression system: Not provided
Length: 5 nucleotides
Theoretical weight: 1.5 KDa
DNA (5'-D(*CP*GP*TP*AP*T)-3') in PDB entry 5tyu, assembly 1, front view.
DNA (5'-D(*CP*GP*TP*AP*T)-3') in PDB entry 5tyu, assembly 1, side view.
DNA (5'-D(*CP*GP*TP*AP*T)-3') in PDB entry 5tyu, assembly 1, top view.
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DNA chain D
Name: DNA (5'-D(P*GP*CP*CP*G)-3')
Representative chains: D
Source organism: unidentified [32644]
Expression system: Not provided
Length: 4 nucleotides
Theoretical weight: 1.19 KDa
DNA (5'-D(P*GP*CP*CP*G)-3') in PDB entry 5tyu, assembly 1, front view.
DNA (5'-D(P*GP*CP*CP*G)-3') in PDB entry 5tyu, assembly 1, side view.
DNA (5'-D(P*GP*CP*CP*G)-3') in PDB entry 5tyu, assembly 1, top view.
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