› 5tvg

X-ray diffraction
2.3Å resolution

Crystal structure of an alpha,alpha-trehalose-phosphate synthase (UDP-forming) from Burkholderia vietnamiensis

Released:
DOI: 10.2210/pdb5tvg/pdb
PDB 5tvg coloured by chain and viewed from the front.
PDB 5tvg coloured by chain and viewed from the side.
PDB 5tvg coloured by chain and viewed from the top.
Source organism: Burkholderia vietnamiensis G4
Entry author: Seattle Structural Genomics Center for Infectious Disease (SSGCID)

Function and Biology Details

Reaction catalysed: 
UDP-alpha-D-glucose + D-glucose 6-phosphate = UDP + alpha,alpha-trehalose 6-phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
homo tetramer
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Trehalose-6-phosphate synthase Chains: A, B, C, D, E, F, G, H
Molecule details ›
Chains: A, B, C, D, E, F, G, H
Length: 481 amino acids
Theoretical weight: 53.86 KDa
Source organism: Burkholderia vietnamiensis G4
Expression system: Escherichia coli
UniProt:
  • Canonical: A4JGS8 (Residues: 1-473; Coverage: 100%)
Gene name: Bcep1808_2483
Sequence domains: Glycosyltransferase family 20
Structure domains: Glycogen Phosphorylase B;

Ligands and Environments

3 bound ligands:
Ligand UDP 8 x UDP
Ligand GOL 13 x GOL
Ligand MG 3 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-F
Spacegroup: P21
Unit cell:
a: 96.17Å b: 103.02Å c: 218.47Å
α: 90° β: 96.42° γ: 90°
R-values:
R R work R free
0.179 0.178 0.224
Expression system: Escherichia coli

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