5tuv

X-ray diffraction
2.9Å resolution

Crystal structure of the E2F5-DP1-p107 ternary complex

Released:

Function and Biology Details

Structure analysis Details

Assemblies composition:
hetero trimer (preferred)
hetero hexamer
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Transcription factor Dp-1 Chains: A, D
Molecule details ›
Chains: A, D
Length: 155 amino acids
Theoretical weight: 17.6 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q14186 (Residues: 199-350; Coverage: 37%)
Gene names: DP1, TFDP1
Sequence domains: Transcription factor DP
Transcription factor E2F5 Chains: B, E
Molecule details ›
Chains: B, E
Length: 112 amino acids
Theoretical weight: 12.96 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q15329 (Residues: 124-232; Coverage: 32%)
Gene name: E2F5
Sequence domains: E2F transcription factor CC-MB domain
Retinoblastoma-like protein 1 Chains: C, F
Molecule details ›
Chains: C, F
Length: 41 amino acids
Theoretical weight: 4.66 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P28749 (Residues: 994-1031; Coverage: 4%)
Gene name: RBL1

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-B
Spacegroup: P21
Unit cell:
a: 60.98Å b: 57.34Å c: 99.198Å
α: 90° β: 96.64° γ: 90°
R-values:
R R work R free
0.215 0.212 0.26
Expression system: Escherichia coli BL21(DE3)