X-ray diffraction
1.55Å resolution

Escherichia coli LigA (K115M) in complex with NAD+


Function and Biology Details

Reaction catalysed:
(1a) NAD(+) + [DNA ligase]-L-lysine = [DNA ligase]-N(6)-(5'-adenylyl)-L-lysine + beta-nicotinamide D-nucleotide
Biochemical function:
Cellular component:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
DNA ligase Chain: A
Molecule details ›
Chain: A
Length: 691 amino acids
Theoretical weight: 76.18 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli BL21(DE3)
  • Canonical: P15042 (Residues: 1-671; Coverage: 100%)
Gene names: JW2403, b2411, dnaL, lig, ligA, lop, pdeC
Sequence domains:

Ligands and Environments

Cofactor: Ligand NAD 1 x NAD
2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: P212121
Unit cell:
a: 40.907Å b: 94.312Å c: 150.927Å
α: 90° β: 90° γ: 90°
R R work R free
0.178 0.176 0.205
Expression system: Escherichia coli BL21(DE3)