X-ray diffraction
2.95Å resolution

Inactive conformation of engineered human cystathionine gamma lyase (E59N, R119L, E339V) to depleting methionine


Function and Biology Details

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Entry contents:
1 distinct polypeptide molecule
Cystathionine gamma-lyase Chains: C, D
Molecule details ›
Chains: C, D
Length: 422 amino acids
Theoretical weight: 46.61 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
  • Canonical: P32929 (Residues: 2-405; Coverage: 100%)
Gene name: CTH
Sequence domains: Cys/Met metabolism PLP-dependent enzyme

Ligands and Environments

1 bound ligand:
1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.3
Spacegroup: P42212
Unit cell:
a: 120.224Å b: 120.224Å c: 125.622Å
α: 90° β: 90° γ: 90°
R R work R free
0.203 0.2 0.254
Expression system: Escherichia coli BL21