PDBe 5to3

X-ray diffraction
2.34Å resolution

Crystal structure of thrombin mutant W215A/E217A fused to EGF456 of thrombomodulin via a 31-residue linker and bound to PPACK

Released:
Source organism: Homo sapiens
Primary publication:
Rational Design of Protein C Activators.
OpenAccess logo Sci Rep 7 44596 (2017)
PMID: 28294177

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Thrombin light chain Chain: A
Molecule details ›
Chain: A
Length: 46 amino acids
Theoretical weight: 5.37 KDa
Source organism: Homo sapiens
Expression system: Cricetulus griseus
UniProt:
  • Canonical: P00734 (Residues: 318-363; Coverage: 8%)
Gene name: F2
Thrombin heavy chain; Thrombomodulin Chain: B
Molecule details ›
Chain: B
Length: 409 amino acids
Theoretical weight: 44.62 KDa
Source organism: Homo sapiens
Expression system: Cricetulus griseus
UniProt:
  • Canonical: P07204 (Residues: 363-483; Coverage: 22%)
  • Canonical: P00734 (Residues: 364-621; Coverage: 43%)
  • nullnull
Gene names: F2, THBD, THRM
Sequence domains: Trypsin

Ligands and Environments


No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007 HF
Spacegroup: C2221
Unit cell:
a: 62.939Å b: 162.174Å c: 128.859Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.216 0.214 0.255
Expression system: Cricetulus griseus