PDBe 5tkz

X-ray diffraction
1.53Å resolution

MEC-8 N-terminal RRM bound to tandem GCAC ligand

Released:

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
Mec-8 protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 94 amino acids
Theoretical weight: 10.32 KDa
Source organism: Caenorhabditis elegans
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: G5ECJ4 (Residues: 28-117; Coverage: 29%)
Gene names: CELE_F46A9.6, F46A9.6, mec-8
Sequence domains: RNA recognition motif. (a.k.a. RRM, RBD, or RNP domain)
DNA (5'-D(*AP*GP*CP*AP*CP*AP*TP*TP*TP*TP*TP*TP*TP*TP*AP*GP*CP*AP*CP*A)-3') Chain: C
Molecule details ›
Chain: C
Length: 20 nucleotides
Theoretical weight: 6.08 KDa
Source organism: unidentified
Expression system: Not provided

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-2
Spacegroup: P3221
Unit cell:
a: 52.188Å b: 52.188Å c: 156.534Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.235 0.233 0.271
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided