› 5tj4

X-ray diffraction
3.5Å resolution

Gasdermin-B C-terminal domain containing the polymorphism residues Gly299:Pro306 fused to maltose binding protein

Released:
DOI: 10.2210/pdb5tj4/pdb
PDB entry 5tj4 coloured by chain, front view.
PDB entry 5tj4 coloured by chain, side view.
PDB entry 5tj4 coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Gene polymorphism linked to increased asthma and IBD risk alters gasdermin-B structure, a sulfatide and phosphoinositide binding protein.
Chao KL, Kulakova L, Herzberg O
Proc Natl Acad Sci U S A 114 E1128-E1137 (2017)
PMID: 28154144

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-186162 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Gasdermin-B, N-terminal Chains: A, B, C, D, E, F, G, H, I, J
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, J
Length: 561 amino acids
Theoretical weight: 61.37 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8TAX9 (Residues: 209-211, 217-415; Coverage: 46%)
Gene names: GSDMB, GSDML, PP4052, PRO2521
Sequence domains: Gasdermin PUB domain

Ligands and Environments

Carbohydrate polymer : NEW Components: GLC
Carbohydrate polymer
1 bound ligand:
Ligand NA 2 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-B
Spacegroup: P21
Unit cell:
a: 89.599Å b: 274.569Å c: 174.019Å
α: 90° β: 96.09° γ: 90°
R-values:
R R work R free
0.215 0.213 0.248
Expression system: Escherichia coli

Quick links

Citations

57 review citations

Gasdermins: Effectors of Pyroptosis.
Kovacs et al. (2017)
56 more

5 mentions without citation

Gene polymorphism linked to increased asthma and IBD risk alters gasdermin-B structure, a sulfatide and phosphoinositide binding protein.
Chao et al. (2017)
4 more