› 5tj3

X-ray diffraction
1.7Å resolution

Crystal structure of wild type alkaline phosphatase PafA to 1.7A resolution

Released:
DOI: 10.2210/pdb5tj3/pdb
PDB entry 5tj3 coloured by chain, front view.
PDB entry 5tj3 coloured by chain, side view.
PDB entry 5tj3 coloured by chain, top view.
Source organism: Elizabethkingia meningoseptica
Primary publication:
Mechanistic and Evolutionary Insights from Comparative Enzymology of Phosphomonoesterases and Phosphodiesterases across the Alkaline Phosphatase Superfamily.
Sunden F, AlSadhan I, Lyubimov AY, Ressl S, Wiersma-Koch H, Borland J, Brown CL, Johnson TA, Singh Z, Herschlag D
J Am Chem Soc 138 14273-14287 (2016)
PMID: 27670607

Function and Biology Details

Reaction catalysed: 
A phosphate monoester + H(2)O = an alcohol + phosphate
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo tetramer
Assembly name:
PDBe Complex ID:
PDB-CPX-191909 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Alkaline phosphatase PafA Chain: A
Molecule details ›
Chain: A
Length: 549 amino acids
Theoretical weight: 61.4 KDa
Source organism: Elizabethkingia meningoseptica
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9KJX5 (Residues: 21-546; Coverage: 100%)
Gene name: pafA
Sequence domains: Type I phosphodiesterase / nucleotide pyrophosphatase
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand ZN 4 x ZN
1 modified residue:
Ligand TPO 1 x TPO

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL11-1
Spacegroup: I4
Unit cell:
a: 113.797Å b: 113.797Å c: 71.442Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.16 0.158 0.177
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Challenges and advances in the computational modeling of biological phosphate hydrolysis.
Petrović et al. (2018)
10 other articles

5 mentions without citation

High throughput and quantitative enzymology in the genomic era.
Mokhtari et al. (2021)
4 more