› 5tgs

X-ray diffraction
2.55Å resolution

Crystal Structure of QueE from Bacillus subtilis with methionine bound

Released:
DOI: 10.2210/pdb5tgs/pdb
PDB entry 5tgs coloured by chain, front view.
PDB entry 5tgs coloured by chain, side view.
PDB entry 5tgs coloured by chain, top view.
Source organism: Bacillus subtilis
Primary publication:
7-Carboxy-7-deazaguanine Synthase: A Radical S-Adenosyl-l-methionine Enzyme with Polar Tendencies.
Bruender NA, Grell TA, Dowling DP, McCarty RM, Drennan CL, Bandarian V
J Am Chem Soc 139 1912-1920 (2017)
PMID: 28045519

Function and Biology Details

Reaction catalysed: 
6-carboxy-5,6,7,8-tetrahydropterin = 7-carboxy-7-carbaguanine + NH(3)
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-128330 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
7-carboxy-7-deazaguanine synthase Chains: A, B
Molecule details ›
Chains: A, B
Length: 263 amino acids
Theoretical weight: 29.32 KDa
Source organism: Bacillus subtilis
Expression system: Escherichia coli
UniProt:
  • Canonical: O31677 (Residues: 1-243; Coverage: 100%)
Gene names: BSU13740, queE, ykvL
Sequence domains:

Ligands and Environments

3 bound ligands:
Ligand SF4 2 x SF4
Ligand MET 1 x MET
Ligand PEG 1 x PEG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.1
Spacegroup: P212121
Unit cell:
a: 54.182Å b: 79.297Å c: 122.224Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.217 0.216 0.248
Expression system: Escherichia coli

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Citations

4 review citations

C-C bond forming radical SAM enzymes involved in the construction of carbon skeletons of cofactors and natural products.
Yokoyama et al. (2018)
3 more