5tbt

X-ray diffraction
2.1Å resolution

Crystal Structure of Isoform 2 of Purine Nucleoside Phosphorylase complexed with Cytidine

Released:

Function and Biology Details

Reaction catalysed:
Purine deoxynucleoside + phosphate = purine + 2'-deoxy-alpha-D-ribose 1-phosphate
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Purine nucleoside phosphorylase Chain: A
Molecule details ›
Chain: A
Length: 287 amino acids
Theoretical weight: 31.36 KDa
Source organism: Schistosoma mansoni
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A0U3AGT1 (Residues: 1-287; Coverage: 100%)
Sequence domains: Phosphorylase superfamily
Structure domains: Nucleoside phosphorylase domain

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04-1
Spacegroup: P213
Unit cell:
a: 100.153Å b: 100.153Å c: 100.153Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.192 0.19 0.226
Expression system: Escherichia coli