5tba

X-ray diffraction
2.49Å resolution

Postcatalytic ternary complex of Human DNA Polymerase Beta with Gapped DNA substrate, incorporated (-)3TC and PPi.

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
1 distinct polypeptide molecule
3 distinct DNA molecules
Macromolecules (4 distinct):
DNA polymerase beta Chain: A
Molecule details ›
Chain: A
Length: 343 amino acids
Theoretical weight: 39.26 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P06746 (Residues: 1-335; Coverage: 100%)
Gene name: POLB
Sequence domains:
Structure domains:
5-MER PHOSPHORYLATED DOWNSTREAM PRIMER Chain: D
Molecule details ›
Chain: D
Length: 5 nucleotides
Theoretical weight: 1.54 KDa
Source organism: synthetic construct
Expression system: Not provided
10-MER PRIMER Chain: P
Molecule details ›
Chain: P
Length: 10 nucleotides
Theoretical weight: 3.06 KDa
Source organism: synthetic construct
Expression system: Not provided
16-MER TEMPLATE Chain: T
Molecule details ›
Chain: T
Length: 16 nucleotides
Theoretical weight: 4.87 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

3 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 31-ID
Spacegroup: P21
Unit cell:
a: 54.697Å b: 79.325Å c: 54.947Å
α: 90° β: 107.03° γ: 90°
R-values:
R R work R free
0.208 0.205 0.273
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided