X-ray diffraction
2Å resolution

Crystal structure of full-length farnesylated and methylated KRAS4b in complex with PDE-delta (crystal form I - with partially disordered hypervariable region)


Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
GTPase KRas Chains: A, C
Molecule details ›
Chains: A, C
Length: 185 amino acids
Theoretical weight: 21.06 KDa
Source organism: Homo sapiens
Expression system: unidentified baculovirus
  • Canonical: P01116 (Residues: 2-186; Coverage: 97%)
  • Best match: P01116-2 (Residues: 2-185)
Gene names: KRAS, KRAS2, RASK2
Sequence domains: Ras family
Structure domains: P-loop containing nucleotide triphosphate hydrolases
Retinal rod rhodopsin-sensitive cGMP 3',5'-cyclic phosphodiesterase subunit delta Chains: B, D
Molecule details ›
Chains: B, D
Length: 150 amino acids
Theoretical weight: 17.44 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
  • Canonical: O43924 (Residues: 1-150; Coverage: 100%)
Gene names: PDE6D, PDED
Sequence domains: GMP-PDE, delta subunit
Structure domains: GMP phosphodiesterase, delta subunit

Ligands and Environments

3 bound ligands:
1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-E
Spacegroup: P21
Unit cell:
a: 58.386Å b: 70.651Å c: 94.307Å
α: 90° β: 92.4° γ: 90°
R R work R free
0.205 0.201 0.248
Expression systems:
  • unidentified baculovirus
  • Escherichia coli