› 5t80

X-ray diffraction
2.62Å resolution

Crystal structure of 10E8 Fab in complex with the MPER epitope scaffold T117v2 and phosphatidic acid (06:0 PA)

Released:
DOI: 10.2210/pdb5t80/pdb
PDB 5t80 coloured by chain and viewed from the front.
PDB 5t80 coloured by chain and viewed from the side.
PDB 5t80 coloured by chain and viewed from the top.
Source organisms:
Primary publication:
Lipid interactions and angle of approach to the HIV-1 viral membrane of broadly neutralizing antibody 10E8: Insights for vaccine and therapeutic design.
Irimia A, Serra AM, Sarkar A, Jacak R, Kalyuzhniy O, Sok D, Saye-Francisco KL, Schiffner T, Tingle R, Kubitz M, Adachi Y, Stanfield RL, Deller MC, Burton DR, Schief WR, Wilson IA
PLoS Pathog 13 e1006212 (2017)
PMID: 28225819

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Antibody 10E8 FAB HEAVY CHAIN Chain: H
Molecule details ›
Chain: H
Length: 236 amino acids
Theoretical weight: 25.45 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
Structure domains: Immunoglobulins
Antibody 10E8 FAB LIGHT CHAIN Chain: L
Molecule details ›
Chain: L
Length: 215 amino acids
Theoretical weight: 22.99 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
Structure domains: Immunoglobulins
10E8 EPITOPE SCAFFOLD T117V2 Chain: G
Molecule details ›
Chain: G
Length: 163 amino acids
Theoretical weight: 18.64 KDa
Source organism: synthetic construct
Expression system: Escherichia coli
Structure domains: Mannitol-specific EII; Chain A

Ligands and Environments

1 bound ligand:
Ligand 44E 1 x 44E
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL12-2
Spacegroup: P212121
Unit cell:
a: 55.221Å b: 67.262Å c: 196.843Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.187 0.184 0.243
Expression systems:
  • Homo sapiens
  • Escherichia coli

Quick links

Citations

7 review citations

Neutralizing Antibodies Targeting HIV-1 gp41.
Caillat et al. (2020)
6 more