5t80

X-ray diffraction
2.62Å resolution

Crystal structure of 10E8 Fab in complex with the MPER epitope scaffold T117v2 and phosphatidic acid (06:0 PA)

Released:

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Antibody 10E8 FAB HEAVY CHAIN Chain: H
Molecule details ›
Chain: H
Length: 236 amino acids
Theoretical weight: 25.45 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
Structure domains: Immunoglobulins
Antibody 10E8 FAB LIGHT CHAIN Chain: L
Molecule details ›
Chain: L
Length: 215 amino acids
Theoretical weight: 22.99 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
Structure domains: Immunoglobulins
10E8 EPITOPE SCAFFOLD T117V2 Chain: G
Molecule details ›
Chain: G
Length: 163 amino acids
Theoretical weight: 18.64 KDa
Source organism: synthetic construct
Expression system: Escherichia coli
Structure domains: Mannitol-specific EII; Chain A

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL12-2
Spacegroup: P212121
Unit cell:
a: 55.221Å b: 67.262Å c: 196.843Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.187 0.184 0.243
Expression systems:
  • Homo sapiens
  • Escherichia coli