X-ray diffraction
1.8Å resolution

Structure of the tryptophan synthase b-subunit from Pyroccus furiosus with b-methyltryptophan non-covalently bound


Function and Biology Details

Reaction catalysed:
(1a) 1-C-(indol-3-yl)glycerol 3-phosphate = indole + D-glyceraldehyde 3-phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Tryptophan synthase beta chain 1 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 396 amino acids
Theoretical weight: 43.89 KDa
Source organism: Pyrococcus furiosus
Expression system: Escherichia coli
  • Canonical: Q8U093 (Residues: 1-388; Coverage: 100%)
Gene names: PF1706, trpB1
Sequence domains: Pyridoxal-phosphate dependent enzyme
Structure domains: Rossmann fold

Ligands and Environments

3 bound ligands:
1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL12-2
Spacegroup: P212121
Unit cell:
a: 82.819Å b: 107.705Å c: 160.061Å
α: 90° β: 90° γ: 90°
R R work R free
0.206 0.204 0.238
Expression system: Escherichia coli