X-ray diffraction
2.3Å resolution

Crystal structure of Aspergillus fumigatus N-myristoyl transferase in complex with myristoyl CoA and a dichloro-dimethylpyridyl-methoxy-phenyl-pyridyl-piperazine ligand


Function and Biology Details

Reaction catalysed:
Tetradecanoyl-CoA + an N-terminal-glycyl-[protein] = CoA + an N-terminal-N-tetradecanoylglycyl-[protein]
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Glycylpeptide N-tetradecanoyltransferase Chain: A
Molecule details ›
Chain: A
Length: 411 amino acids
Theoretical weight: 47.44 KDa
Source organism: Aspergillus fumigatus Af293
Expression system: Escherichia coli BL21(DE3)
  • Canonical: Q9UVX3 (Residues: 86-492; Coverage: 83%)
Gene names: AFUA_4G08070, nmt1
Sequence domains:
Structure domains: Aminopeptidase

Ligands and Environments

Cofactor: Ligand MYA 1 x MYA
1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-1
Spacegroup: P212121
Unit cell:
a: 51.276Å b: 58.339Å c: 152.1Å
α: 90° β: 90° γ: 90°
R R work R free
0.199 0.196 0.248
Expression system: Escherichia coli BL21(DE3)