› 5t5o

X-ray diffraction
2.75Å resolution

LECTIN FROM BAUHINIA FORFICATA IN COMPLEX WITH TN-PEPTIDE

Released:
DOI: 10.2210/pdb5t5o/pdb
PDB entry 5t5o coloured by chain, front view.
PDB entry 5t5o coloured by chain, side view.
PDB entry 5t5o coloured by chain, top view.
Source organisms:
Primary publication:
Structural analysis and unique molecular recognition properties of a Bauhinia forficata lectin that inhibits cancer cell growth.
Lubkowski J, Durbin SV, Silva MC, Farnsworth D, Gildersleeve JC, Oliva ML, Wlodawer A
FEBS J 284 429-450 (2017)
PMID: 27973758

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-160992 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Lectin Chains: A, B, C, D, E, F, G, H, I, J
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, J
Length: 242 amino acids
Theoretical weight: 27.14 KDa
Source organism: Bauhinia forficata
Expression system: Escherichia coli
UniProt:
  • Canonical: P86993 (Residues: 1-233; Coverage: 100%)
Sequence domains: Legume lectin domain
Structure domains: Jelly Rolls
TN-peptide ACE-GLY-VAL-THR-SER-ALA Chains: a, b, c, d, e, f, g, h, i, j
Molecule details ›
Chains: a, b, c, d, e, f, g, h, i, j
Length: 6 amino acids
Theoretical weight: 459 Da
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

2 bound ligands:
Ligand CA 20 x CA
Ligand A2G 10 x A2G
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P212121
Unit cell:
a: 65.081Å b: 187.29Å c: 258.387Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.203 0.201 0.23
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

5 review citations

Plant Lectins Targeting O-Glycans at the Cell Surface as Tools for Cancer Diagnosis, Prognosis and Therapy.
Poiroux et al. (2017)
4 more

2 mentions without citation

Molecular Recognition of GalNAc in Mucin-Type O-Glycosylation.
Sanz-Martinez et al. (2023)
1 more