5t5g

X-ray diffraction
2.1Å resolution

human SETD8 in complex with MS2177

Released:

Function and Biology Details

Reaction catalysed:
S-adenosyl-L-methionine + a [histone H4]-L-lysine(20) = S-adenosyl-L-homocysteine + a [histone H4]-N(6)-methyl-L-lysine(20)
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
N-lysine methyltransferase KMT5A Chain: A
Molecule details ›
Chain: A
Length: 148 amino acids
Theoretical weight: 16.78 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q9NQR1 (Residues: 234-380; Coverage: 37%)
Gene names: KMT5A, PRSET7, SET07, SET8, SETD8
Sequence domains: SET domain
Structure domains: SET domain

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P3221
Unit cell:
a: 88.957Å b: 88.957Å c: 34.989Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.206 0.205 0.227
Expression system: Escherichia coli BL21