› 5t59

X-ray diffraction
2.4Å resolution

Structure of the MIND Complex Shows a Regulatory Focus of Yeast Kinetochore Assembly

Released:
DOI: 10.2210/pdb5t59/pdb
PDB entry 5t59 coloured by chain, front view.
PDB entry 5t59 coloured by chain, side view.
PDB entry 5t59 coloured by chain, top view.
Source organism: Kluyveromyces lactis NRRL Y-1140
Primary publication:
Structure of the MIND Complex Defines a Regulatory Focus for Yeast Kinetochore Assembly.
Dimitrova YN, Jenni S, Valverde R, Khin Y, Harrison SC
Cell 167 1014-1027.e12 (2016)
PMID: 27881300

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-179450 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Mtw1 Chains: A, D
Molecule details ›
Chains: A, D
Length: 121 amino acids
Theoretical weight: 13.87 KDa
Source organism: Kluyveromyces lactis NRRL Y-1140
Expression system: Escherichia coli
UniProt:
  • Canonical: Q6CLK3 (Residues: 2-120; Coverage: 41%)
Gene name: KLLA0_F02343g
Sequence domains: Mis12 protein
Kinetochore-associated protein Chains: B, E
Molecule details ›
Chains: B, E
Length: 102 amino acids
Theoretical weight: 12.1 KDa
Source organism: Kluyveromyces lactis NRRL Y-1140
Expression system: Escherichia coli
UniProt:
  • Canonical: Q6CPD1 (Residues: 1-102; Coverage: 50%)
Gene name: KLLA0_E05809g
Sequence domains: Nnf1
Mif2/CENP-C cupin domain-containing protein Chains: C, F
Molecule details ›
Chains: C, F
Length: 41 amino acids
Theoretical weight: 4.83 KDa
Source organism: Kluyveromyces lactis NRRL Y-1140
Expression system: Escherichia coli
UniProt:
  • Canonical: Q6CVA1 (Residues: 1-41; Coverage: 7%)
Gene name: KLLA0_B13629g
Sequence domains: Kinetochore CENP-C fungal homologue, Mif2, N-terminal

Ligands and Environments

1 bound ligand:
Ligand NHE 2 x NHE
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: P212121
Unit cell:
a: 75.4Å b: 91.7Å c: 95.57Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.251 0.249 0.29
Expression system: Escherichia coli

Quick links

Citations

16 review citations

A Molecular View of Kinetochore Assembly and Function.
Musacchio et al. (2017)
15 more

1 mention without citation

Structure of the MIND Complex Defines a Regulatory Focus for Yeast Kinetochore Assembly.
Dimitrova et al. (2016)