› 5t54

X-ray diffraction
1.65Å resolution

LECTIN FROM BAUHINIA FORFICATA IN COMPLEX WITH BLOOD GROUP A ANTIGEN

Released:
DOI: 10.2210/pdb5t54/pdb
PDB entry 5t54 coloured by chain, front view.
PDB entry 5t54 coloured by chain, side view.
PDB entry 5t54 coloured by chain, top view.
Source organism: Bauhinia forficata
Primary publication:
Structural analysis and unique molecular recognition properties of a Bauhinia forficata lectin that inhibits cancer cell growth.
Lubkowski J, Durbin SV, Silva MC, Farnsworth D, Gildersleeve JC, Oliva ML, Wlodawer A
FEBS J 284 429-450 (2017)
PMID: 27973758

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-160994 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Lectin Chains: A, B
Molecule details ›
Chains: A, B
Length: 242 amino acids
Theoretical weight: 27.14 KDa
Source organism: Bauhinia forficata
Expression system: Escherichia coli
UniProt:
  • Canonical: P86993 (Residues: 1-233; Coverage: 100%)
Sequence domains: Legume lectin domain
Structure domains: Jelly Rolls

Ligands and Environments

Carbohydrate polymer : NEW Components: GLA, FUC, A2G
Carbohydrate polymer
2 bound ligands:
Ligand CA 4 x CA
Ligand EDO 4 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P21
Unit cell:
a: 78.829Å b: 44.82Å c: 65.771Å
α: 90° β: 100.05° γ: 90°
R-values:
R R work R free
0.151 0.15 0.177
Expression system: Escherichia coli

Quick links

Citations

6 review citations

Plant Lectins Targeting O-Glycans at the Cell Surface as Tools for Cancer Diagnosis, Prognosis and Therapy.
Poiroux et al. (2017)
5 more

1 mention without citation

Structural analysis and unique molecular recognition properties of a Bauhinia forficata lectin that inhibits cancer cell growth.
Lubkowski et al. (2017)