› 5t4l

X-ray diffraction
1.53Å resolution

PLP and GABA Trigger GabR-Mediated Transcription Regulation in Bacillus subsidies via External Aldimine Formation

Released:
DOI: 10.2210/pdb5t4l/pdb
PDB entry 5t4l coloured by chain, front view.
PDB entry 5t4l coloured by chain, side view.
PDB entry 5t4l coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
PLP and GABA trigger GabR-mediated transcription regulation in Bacillus subtilis via external aldimine formation.
Wu R, Sanishvili R, Belitsky BR, Juncosa JI, Le HV, Lehrer HJ, Farley M, Silverman RB, Petsko GA, Ringe D, Liu D
Proc Natl Acad Sci U S A 114 3891-3896 (2017)
PMID: 28348215

Function and Biology Details

Reaction catalysed: 
L-aspartate + 2-oxoglutarate = oxaloacetate + L-glutamate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132575 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Aspartate aminotransferase Chain: A
Molecule details ›
Chain: A
Length: 396 amino acids
Theoretical weight: 43.62 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P00509 (Residues: 1-396; Coverage: 100%)
Gene names: JW0911, aspC, b0928
Sequence domains: Aminotransferase class I and II
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand 77E 1 x 77E
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-D
Spacegroup: C2221
Unit cell:
a: 84.913Å b: 154.916Å c: 79.342Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.15 0.149 0.166
Expression system: Escherichia coli

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Citations

3 review citations

Role of the Extremolytes Ectoine and Hydroxyectoine as Stress Protectants and Nutrients: Genetics, Phylogenomics, Biochemistry, and Structural Analysis.
Czech et al. (2018)
2 more

1 mention without citation

PLP and GABA trigger GabR-mediated transcription regulation in Bacillus subtilis via external aldimine formation.
Wu et al. (2017)