› 5t3j

X-ray diffraction
2.55Å resolution

Histidinol Phosphate Phosphatase(HPP) soaked with selenourea for 10 min

Released:
DOI: 10.2210/pdb5t3j/pdb
PDB entry 5t3j coloured by chain, front view.
PDB entry 5t3j coloured by chain, side view.
PDB entry 5t3j coloured by chain, top view.
Source organism: Medicago truncatula
Primary publication:
Selenourea: a convenient phasing vehicle for macromolecular X-ray crystal structures.
Luo Z
Sci Rep 6 37123 (2016)
PMID: 27841370

Function and Biology Details

Reaction catalysed: 
L-histidinol phosphate + H(2)O = L-histidinol + phosphate
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-124572 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
histidinol-phosphatase Chain: A
Molecule details ›
Chain: A
Length: 277 amino acids
Theoretical weight: 30.41 KDa
Source organism: Medicago truncatula
Expression system: Escherichia coli
UniProt:
  • Canonical: G7J7Q5 (Residues: 52-326; Coverage: 84%)
Gene names: 11419457, MTR_3g117220, MtrunA17_Chr3g0145001
Sequence domains: Inositol monophosphatase family
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand SEY 1 x SEY
Ligand PO4 1 x PO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-BM
Spacegroup: P6222
Unit cell:
a: 119.635Å b: 119.635Å c: 92.906Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.207 0.204 0.237
Expression system: Escherichia coli

Quick links

Citations

10 citation in other articles

Chalcogen Bonds Involving Selenium in Protein Structures.
Carugo et al. (2021)
9 more

1 mention without citation

Selenourea: a convenient phasing vehicle for macromolecular X-ray crystal structures.
Luo Z. (2016)