› 5t0o

X-ray diffraction
3.15Å resolution

Crystal Structure of a membrane protein

Released:
DOI: 10.2210/pdb5t0o/pdb
PDB entry 5t0o coloured by chain, front view.
PDB entry 5t0o coloured by chain, side view.
PDB entry 5t0o coloured by chain, top view.
Source organism: Campylobacter jejuni
Primary publication:
Structures and transport dynamics of a Campylobacter jejuni multidrug efflux pump.
Su CC, Yin L, Kumar N, Dai L, Radhakrishnan A, Bolla JR, Lei HT, Chou TH, Delmar JA, Rajashankar KR, Zhang Q, Shin YK, Yu EW
Nat Commun 8 171 (2017)
PMID: 28761097

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-186043 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
SSD domain-containing protein Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 1040 amino acids
Theoretical weight: 114.06 KDa
Source organism: Campylobacter jejuni
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8RTE4 (Residues: 1-1040; Coverage: 100%)
Gene names: NCTC13105_01092, QQI97_001037, bepE, cmeB
Sequence domains: AcrB/AcrD/AcrF family
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-E
Spacegroup: C2
Unit cell:
a: 300.71Å b: 147.538Å c: 120.027Å
α: 90° β: 99.87° γ: 90°
R-values:
R R work R free
0.214 0.211 0.268
Expression system: Escherichia coli

Quick links

Citations

11 review citations

Multidrug efflux pumps: structure, function and regulation.
Du et al. (2018)
10 more

6 mentions without citation

Structural and Functional Diversity of Resistance-Nodulation-Cell Division Transporters.
Klenotic et al. (2021)
5 more