› 5t0b

X-ray diffraction
2Å resolution

Crystal structure of H6 hemagglutinin G225D mutant from Taiwan (2013) H6N1 influenza virus in complex with 6'-SLN

Released:
DOI: 10.2210/pdb5t0b/pdb
PDB entry 5t0b coloured by chain, front view.
PDB entry 5t0b coloured by chain, side view.
PDB entry 5t0b coloured by chain, top view.
Source organism: H6N1 subtype
Primary publication:
A single mutation in Taiwanese H6N1 influenza hemagglutinin switches binding to human-type receptors.
de Vries RP, Tzarum N, Peng W, Thompson AJ, Ambepitiya Wickramasinghe IN, de la Pena ATT, van Breemen MJ, Bouwman KM, Zhu X, McBride R, Yu W, Sanders RW, Verheije MH, Wilson IA, Paulson JC
EMBO Mol Med 9 1314-1325 (2017)
PMID: 28694323

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-102268 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (3 distinct):
Hemagglutinin Chains: A, C, E
Molecule details ›
Chains: A, C, E
Length: 333 amino acids
Theoretical weight: 37.39 KDa
Source organism: H6N1 subtype
Expression system: Trichoplusia ni
UniProt:
  • Canonical: A0A0J9X268 (Residues: 1-333; Coverage: 100%)
Gene name: HA
Sequence domains: Haemagglutinin
Structure domains:
Hemagglutinin HA2 chain Chains: B, D, F
Molecule details ›
Chains: B, D, F
Length: 180 amino acids
Theoretical weight: 20.63 KDa
Source organism: H6N1 subtype
Expression system: Trichoplusia ni
UniProt:
  • Canonical: A0A0J9X267 (Residues: 1-180; Coverage: 100%)
Gene name: HA
Sequence domains: Haemagglutinin
Structure domains: Hemagglutinin Ectodomain; Chain B

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, GAL, SIA
Carbohydrate polymer
1 bound ligand:
Ligand NAG 5 x NAG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-B
Spacegroup: C2
Unit cell:
a: 185.522Å b: 98.927Å c: 133.664Å
α: 90° β: 126.63° γ: 90°
R-values:
R R work R free
0.215 0.214 0.246
Expression system: Trichoplusia ni

Quick links

Citations

11 review citations

Inventory of molecular markers affecting biological characteristics of avian influenza A viruses.
Suttie et al. (2019)
10 more

2 mentions without citation

A single mutation in Taiwanese H6N1 influenza hemagglutinin switches binding to human-type receptors.
de Vries et al. (2017)
1 more