5t05

X-ray diffraction
1.95Å resolution

Crystal structure of heparan sulfate 6-O-sulfotransferase with bound PAP and IdoA2S containing hexasaccharide substrate

Released:
Source organism: Danio rerio

Function and Biology Details

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (3 distinct):
Maltose/maltodextrin-binding periplasmic protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 692 amino acids
Theoretical weight: 78.56 KDa
Source organism: Danio rerio
Expression system: Escherichia coli
UniProt:
  • Canonical: P0AEX9 (Residues: 27-392; Coverage: 99%)
Gene names: JW3994, b4034, malE
Sequence domains: Bacterial extracellular solute-binding protein

Ligands and Environments

Carbohydrate polymer : NEW Components: BDP, GNS, IDS
Carbohydrate polymer : NEW Components: GLC
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P212121
Unit cell:
a: 75.785Å b: 128.161Å c: 178.711Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.179 0.178 0.212
Expression system: Escherichia coli